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Plasmid#224518PurposeA Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)DepositorInsertmTurquoise-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME iCre-I (JDW 1203)
Plasmid#224520PurposeA Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteriaDepositorInsertiCre-I
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2A_mCherry_SV40pA (JDW 967)
Plasmid#224528PurposeA Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyADepositorInsertH2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ark-L2
Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB425
Plasmid#74819PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-C-EGFP
Plasmid#122844PurposeC-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-mCherry
Plasmid#123215PurposeN-terminal mCherry tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP
Plasmid#11085DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromotertac promoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-EGFP
Plasmid#122842PurposeN-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSDONR P4r-P2
Plasmid#121527PurposeGateway Entry Vector for Multisite Gateway CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-VPS35
Plasmid#166220PurposeGateway entry vector for N. furzeri VPS35DepositorArticleInsertVPS35
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-AAK1_IDG-K
Plasmid#135280PurposeGateway destination clone of AAK1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-AAK1 (AAK1 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-STK32C_IDG-K
Plasmid#170610PurposeGateway destination clone of STK32C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-STK32C (STK32C Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-TP53RK_IDG-K
Plasmid#170589PurposeGateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-TP53RK (TP53RK Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Neo
Plasmid#48993PurposeGateway adapted transfer of Neomycin resistance geneDepositorInsertaminoglycoside 3' phosphotransferase
UseGateway attb1 & attb2TagsExpressionMutationPromoterAvailable sinceApril 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p667-UBC-AAK1-V5_IDG-K
Plasmid#135257PurposeGateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAAK1-V5 (AAK1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only