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Plasmid#183647PurposeLentiviral expression of a mitochondrion-residential PEN2.DepositorUseLentiviralTagsExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pBoBi-hLAMP2-C-PEN2
Plasmid#183646PurposeLentiviral expression of a lysosome-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65429PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Dre Flp-in replacement vector
Plasmid#61571PurposeRecombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codonDepositorInsertPvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 1-419
Plasmid#183644PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only amino aci…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1 Δ420-440
Plasmid#183642PurposeTo (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseLentiviralTagsHAExpressionMutationtruncated ATP6AP1 mutant lacking the transmembran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pattB-LSL-AAVR-F2A-spCas9
Plasmid#202459PurposeThe plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.DepositorInsertAAVR (AU040320 Mouse)
UseCRISPR, Cre/Lox, and Mouse TargetingTagsmCherry fusionExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 420-440
Plasmid#183643PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only the trans…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1-C-LAMP2 TM
Plasmid#183645PurposeLentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.DepositorUseLentiviralTagsHAExpressionMutationATP6AP1 transmembrane domain replaced by the tran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-HA
Plasmid#63681Purposeadenoviral shuttle vector containing N-terminal Flag tagDepositorTypeEmpty backboneUseAdenoviralTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pANY4
Plasmid#164897PurposeTwo valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY8
Plasmid#164900PurposeContaining His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.DepositorInsertccdB cassette
UseTagsHis-ELP-Intein-tagExpressionBacterialMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Beta
Plasmid#177962PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)DepositorInsertSARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
UseTagsExpressionMammalianMutationK417N, E484K, N501Y, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Epsilon
Plasmid#177964PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)DepositorInsertSARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
UseTagsExpressionMammalianMutationS13I, W152C, L452R, D614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-K417N
Plasmid#177969PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)DepositorInsertSARS-CoV-2 Spike protein (D614G, K417N) (S )
UseTagsExpressionMammalianMutationD614G, K417NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N439K
Plasmid#177970PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)DepositorInsertSARS-CoV-2 Spike protein (D614G, N439K) (S )
UseTagsExpressionMammalianMutationD614G, N439KPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-L452R
Plasmid#177971PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)DepositorInsertSARS-CoV-2 Spike protein (D614G, L452R) (S )
UseTagsExpressionMammalianMutationD614G, L452RPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-S477N
Plasmid#177972PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)DepositorInsertSARS-CoV-2 Spike protein (D614G, S477N) (S )
UseTagsExpressionMammalianMutationD614G, S477NPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N501Y
Plasmid#177975PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)DepositorInsertSARS-CoV-2 Spike protein (D614G, N501Y) (S )
UseTagsExpressionMammalianMutationD614G, N501YPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-S13I
Plasmid#177965PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)DepositorInsertSARS-CoV-2 Spike protein (D614G, S13I) (S )
UseTagsExpressionMammalianMutationD614G, S13IPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only