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Addgene
Showing: 1521 - 1540 of 3192 results

  1. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Beta actin
    Availability
    Academic Institutions and Nonprofits only
    134606-plasmid-map-sequence-id-258758

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  2. pUAS-kkv-OH

    Plasmid
    #138956
    Purpose
    Used to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv with C terminal epitope tag (kkv Fly)
    Use
    Tags
    ollas and his-6 added to C terminus of protein co…
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Availability
    Academic Institutions and Nonprofits only
    138956-plasmid-map-sequence-id-274764

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  3. pHD-DsRed-kkv-3::NG

    Plasmid
    #138960
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    3' end of kkv and neon green (kkv Fly)
    Use
    An editing vector for drosophila
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138960-plasmid-map-sequence-id-274768

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  4. human ACF

    Plasmid
    #112860
    Purpose
    plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 complementation factor (A1CF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112860-plasmid-map-sequence-id-224730

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  5. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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  7. EB3-3xmTq2-N1

    Plasmid
    #112929
    Purpose
    In vivo visualization of microtuble plus ends (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    EB3
    Use
    Tags
    3x mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    112929-plasmid-map-sequence-id-223579

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  10. NODALvar-MYC-DYK-N328A

    Plasmid
    #115246
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N328A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    115246-plasmid-map-sequence-id-222166

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  14. NODALvar-CAG-AAVS1

    Plasmid
    #115262
    Purpose
    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    115262-plasmid-map-sequence-id-222505

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  17. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AND-gate and pLacI
    Availability
    Academic Institutions and Nonprofits only
    112237-plasmid-map-sequence-id-218819

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  20. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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Showing: 1521 - 1540 of 3192 results