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pDONR223_PIK3IP1_WT

Plasmid

#81853

Purpose

Gateway Donor vector containing PIK3IP1 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T251S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G185E; T251S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.R171W

Plasmid

#81592

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R171W

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.E4K

Plasmid

#82860

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, David Root, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E4K; E849D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, David Root, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y580F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC-GFP-IRES-mCherry

Plasmid

#231232

Purpose

MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MCRS1-GFP-IRES-mCherry

Plasmid

#231231

Purpose

MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-F97W

Plasmid

#203572

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Phenylalanine 97 to Tryptophan for partia…

Promoter

CMV

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-Embigin

Plasmid

#221417

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

Embigin (EMB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209062

Purpose

Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

non-targeting sgRNA (C0111)

Use

Gateway vector to be used for lr reaction

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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