-
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-QUASM1
Plasmid#155114Purpose5' entry vector containing QUASM1 promoter element.DepositorInsertQUASM1
UseGateway 5' entry vectorTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP
Plasmid#75175PurposeTagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertTagBFP
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSYC-97
Plasmid#31565DepositorInsertpCS4+-NLS-EGFP-P2A-mCherry-CAAX
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
1456_pDEST_miniTol2_R4-R2_Cryst-eGFP
Plasmid#171795PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-and1 in situ probe
Plasmid#52102Purposeactinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 polDepositorInsertactinodin 1 in situ probe (and1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-QUAS5x
Plasmid#155113Purpose5' entry vector containing QUAS5x promoter element.DepositorInsertQUAS5x
UseGateway 5' entry vectorTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorInsertsUseGatewayTags2A-mCherryExpressionMutationPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-P1Egfp
Plasmid#90148Purposecontains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aE1P1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-LAMP1-mRFP
Plasmid#169004PurposeExpress rat LAMP1-mRFP in zebrafishDepositorInsertrat LAMP1-mRFP
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-LAMP1-EGFP
Plasmid#169003PurposeExpress rat LAMP1-EGFP in zebrafishDepositorInsertrat LAMP1-EGFP
UseUnspecifiedTagsEGFPExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ MBIC5-mCer3
Plasmid#74116PurposeFor generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)DepositorInsertMBIC5-mCer3
UseTransgenic constructTagsmCerulean 3ExpressionMutationPromoterMyl7 (Cmlc2)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-slc6a3[11kb]:egfp
Plasmid#53240Purposeallow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthoodDepositorInsertDAT-GFP
UseCloningTagsGFPExpressionMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
actb2-lifeact-mKate2
Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HybridBarcode
Plasmid#174037PurposeFor Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sitesDepositorInsertCRISPR target sites
UseTol2 transgenesisTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only