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Plasmid#76082Purpose3rd generation lentiviral gRNA plasmid targeting human ALPK1DepositorInsertALPK1 (ALPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT1 E3.4 gRNA
Plasmid#90842Purpose3rd generation lentiviral gRNA plasmid targeting human POT1DepositorInsertPOT1 (Guide Designation E3.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
WEE1 F10.4 gRNA
Plasmid#90941Purpose3rd generation lentiviral gRNA plasmid targeting human WEE1DepositorInsertWEE1 (Guide Designation F10.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
WEE1 F9.4 gRNA
Plasmid#90940Purpose3rd generation lentiviral gRNA plasmid targeting human WEE1DepositorInsertWEE1 (Guide Designation F9.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-P3-evoCjCas9-eAID_gRNA
Plasmid#202562PurposeP3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)DepositorInsertevoCjCas9-eAID
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2ABFP
Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EPHA2 gRNA (BRDN0001146397)
Plasmid#76367Purpose3rd generation lentiviral gRNA plasmid targeting human EPHA2DepositorInsertEPHA2 (EPHA2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K14 gRNA (BRDN0001144843)
Plasmid#77736Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K14DepositorInsertMAP3K14 (MAP3K14 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK4 gRNA (BRDN0001145730)
Plasmid#76176Purpose3rd generation lentiviral gRNA plasmid targeting human CDK4DepositorInsertCDK4 (CDK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
ALPK3 gRNA (BRDN0001145912)
Plasmid#77324Purpose3rd generation lentiviral gRNA plasmid targeting human ALPK3DepositorInsertALPK3 (ALPK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ALPK3 gRNA (BRDN0001146913)
Plasmid#77325Purpose3rd generation lentiviral gRNA plasmid targeting human ALPK3DepositorInsertALPK3 (ALPK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FOXM1 C11.2 gRNA
Plasmid#90690Purpose3rd generation lentiviral gRNA plasmid targeting human FOXM1DepositorInsertFOXM1 (Guide Designation C11.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-xCas9-sgRNA
Plasmid#179579PurposeExpresses xCas9 and gRNA that targets ColE1 plasmids for curing.DepositorInsertxCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PFKFB3 gRNA (BRDN0001146230)
Plasmid#76462Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB3DepositorInsertPFKFB3 (PFKFB3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD4 gRNA (BRDN0001146786)
Plasmid#77346Purpose3rd generation lentiviral gRNA plasmid targeting human BRD4DepositorInsertBRD4 (BRD4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - DDX3Y sgRNA 2
Plasmid#70657PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against DDX3Y
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T3_gRNA
Plasmid#199335PurposepDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNADepositorInsertszCREST3
Cas9
nrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ROCK1 gRNA (BRDN0001147709)
Plasmid#77593Purpose3rd generation lentiviral gRNA plasmid targeting human ROCK1DepositorInsertROCK1 (ROCK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hgRNA-D21_pLKO-Hyg
Plasmid#100562PurposehgRNA-D21 in a lentiviral backboneDepositorInserthgRNA-D21
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only