Showing: 3751 - 3800 of 144353 results
-
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN42
Plasmid#61345PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS10 and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN43
Plasmid#61346PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS11 and PKIt NESExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
Plasmid#61359Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (C1204R)
Plasmid#61361Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; C1204R mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1396/1397 SY/WW)
Plasmid#61363Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL G457D
Plasmid#61367PurposeExpresses Human full length RIPK3 with G457Dmutation in the mammalian cellsDepositorInsertFull length human RIP3 with G457D mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged G457 to DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL VV458/460PP
Plasmid#61368PurposeExpresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with VV458/460P mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to P and V460to PPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL N464D
Plasmid#61369PurposeExpresses Human full length RIPK3 with N464D mutation in the mammalian cellsDepositorInsertFull length human RIP3 with N464D mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged N464to DPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS (L4-R1)
Plasmid#61372Purpose5' Gateway Entry Clone Containing 4xnrUASDepositorInsert4xnrUAS
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:GFP
Plasmid#61373Purpose4xnrUAS driving GFPDepositorInsert4xnr UAS:GFP
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p5E-QUAS (L4-R1)
Plasmid#61374Purpose5' Gateway Entry Vector Containing QUASDepositorInsertQUAS
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pME-QF
Plasmid#61375PurposeMiddle Gateway Entry Vector Containing QFDepositorInsertQF
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V458P
Plasmid#61377PurposeExpresses Human full length RIPK3 with V458P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V458Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to PPromoterAvailabilityAcademic Institutions and Nonprofits only -
pME-QS (L1-L2)
Plasmid#61378PurposeGateway Middle Entry Vector Containing QSDepositorInsertQS
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL V460P
Plasmid#61379PurposeExpresses Human full length RIPK3 with V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with V460Pmutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V460 to PPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I539P
Plasmid#61380PurposeExpresses Human full length RIPK1with I539P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I539P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I539 to PPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R2
Plasmid#61383PurposeExpresses TRAIL receptor 2DepositorInsertTRAIL receptor 2 (TNFRSF10B Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TRAIL-R3
Plasmid#61384PurposeExpresses TRAIL receptor 3DepositorInsertTRAIL receptor 3 (TNFRSF10C Human)
UseTagsHA tag and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R3 leader peptide (aa1-29) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R4
Plasmid#61385PurposeExpresses HA tagged TRAIL receptor 4DepositorInsertTRAIL receptor 4 (TNFRSF10D Human)
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R4 leader peptide (aa1-29) replaced with TN…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRIP3-mCherry
Plasmid#61386PurposeExpresses RIP3-mCherry in mammalian cellsDepositorInsertRIP3 (RIPK3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2-NfsB.zf1
Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionTagsExpressionMutationE28GPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tol1.zf1
Plasmid#61388PurposemRNA synthesis of tol1 transposase codon modified for zebrafishDepositorInserttol1.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6AvailabilityAcademic Institutions and Nonprofits only -
pBS-Isce1-eno2:egfp
Plasmid#61394PurposeExpresses GFP in neurons of transgenic zebrafishDepositorInserteno2 regulatory element
UseZebrafish expressionTagsExpressionMutationPromotereno2AvailabilityAcademic Institutions and Nonprofits only -
pL-SFFV.Reporter.RFP657.PAC
Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pEGFP_C1 I3
Plasmid#61397PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3
Plasmid#61398PurposeExpresses mCherry-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3(resist)
Plasmid#61399PurposeExpresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(resist)
Plasmid#61400PurposeExpresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3
Plasmid#61401PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(V41A/W43A)
Plasmid#61402PurposeExpresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cellsDepositorInsertinhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationV41A/W43APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNeuroD-ires-GFP
Plasmid#61403PurposeMammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequenceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNeuroDAvailabilityAcademic Institutions and Nonprofits only
Showing: 3751 - 3800 of 144353 results