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Showing: 3001 - 3040 of 156611 results

  1. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid
    #208573
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    mnucTomato
    d2nucEGFP
    HygroR
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/CMV and minP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208573-plasmid-map-sequence-id-412948

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  2. ADRB3-DuET

    Plasmid
    #213182
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB3 (ADRB3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213182-plasmid-map-sequence-id-430749

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  8. pD649-HAsp-MERTK-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156878
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MERTK (MERTK Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156878-plasmid-map-sequence-id-382010

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  12. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  13. pET21a-alpha-synuclein

    Plasmid
    #51486
    Purpose
    Inducible expression of human alpha-synuclein in E.coli
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    51486-plasmid-map-sequence-id-139447

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  19. pDule2-Mb haloTyrRS C6

    Plasmid
    #160378
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160378-plasmid-map-sequence-id-315318

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  22. pQE81XN-mStayGold(J)

    Plasmid
    #225445
    Purpose
    Expresses Hisx6-Tagged mStayGold(J) in bacteria
    Depositor
    Benjamin Glick
    Insert
    mStayGold(J)
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225445-plasmid-map-sequence-id-446225

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  23. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  28. mCherry-LaminB1-10

    Plasmid
    #55069
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55069-plasmid-map-sequence-id-204489

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  39. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

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Showing: 3001 - 3040 of 156611 results