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Plasmid#214300PurposeExpress constitutively active KRas fused with MBPDepositorInsertConstitutively active KRas (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-KRas-S17N
Plasmid#214301PurposeExpress dominant negative KRas fused with MBPDepositorInsertDominant negative KRas (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-S17NPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223.1_mmDdx58 (closed)
Plasmid#160107PurposeExpresses murine Ddx58 in mammalian cellsDepositorInsertDdx58 (Rigi Mouse)
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA
Plasmid#177345PurposeAAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoterDepositorInsertdead hSaCas9
UseAAV and CRISPRTags3x GCN4 peptide, 3xHA, and NLSExpressionMammalianMutationD10A, N580APromoterSynapsinAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_3xFLAG_V5_ mmDdx58_V5
Plasmid#160108PurposeExpresses murine Ddx58 in mammalian cellsDepositorInsertDdx58 (Rigi Mouse)
UseLentiviralTags3xFLAG, V5ExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…TagsExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero20
Plasmid#187930PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero20DepositorInsertHero20 (C11orf58 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA
Plasmid#177342PurposeAAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequenceDepositorInsertdead hSaCas9
UseAAV and CRISPRTags3x GCN4 peptide, 3xHA, and NLSExpressionMammalianMutationD10A, N580APromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-FRB-KRas/N12
Plasmid#214302PurposeExpress split-KRas fragment fused with FRB and MBPDepositorInsertSplit-KRas fragment fused with FRB (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-KRas/13C-FKBP-MBP
Plasmid#214303PurposeExpress split-KRas fragment fused with FKBP and MBPDepositorInsertSplit-KRas fragment fused with FKBP (KRAS Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationKRas-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-1899)
Plasmid#202514PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-706/-277)
Plasmid#202511PurposeExpressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-254)
Plasmid#202512PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-(-706)
Plasmid#202513PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)DepositorInsertACSL4 promoter (partial) (ACSL4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
Plasmid#162278PurposeEukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN1A Variant 1, stabilized (SCN1A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp2946. Silent codon c…PromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmIrf1
Plasmid#160098PurposeExpresses murine Irf1 in mammalian cellsDepositorInsertIrf1 (Irf1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmMavs_V5
Plasmid#160100PurposeExpresses murine Mavs in mammalian cellsDepositorInsertMavs (Mavs Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_mmIrf1 (closed)
Plasmid#160097PurposeExpresses murine Irf1 in mammalian cellsDepositorInsertIrf1 (Irf1 Mouse)
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only