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Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-F>E
Plasmid#185508PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionTagsExpressionMutationF>EPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-foxd4l1.1-A6
Plasmid#185506PurposeExpression of Xenopus laevis foxd4DepositorInsertfoxd4l1.1
UseIn vitro transcriptionTagsExpressionMutationFSIENIM to AAAAAAMPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-pumilioCENP-C
Plasmid#174446Purposein vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNArodA
Plasmid#149658Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9H(-10TC)_arr2
Plasmid#149587Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAflaB
Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PflaBS-sgRNAflaB
Plasmid#149643Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PflaBSAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_arr2
Plasmid#149582Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826L-sgRNAflaB
Plasmid#149648Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0826LAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNArodA
Plasmid#149655Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTS-sgRNAflaB
Plasmid#149644Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTL-sgRNAflaB
Plasmid#149645Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTLAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0026-sgRNAflaB
Plasmid#149646Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0026Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0826S-sgRNAflaB
Plasmid#149647Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0826SAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAftsI
Plasmid#149649Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNAftsI
Plasmid#149650Purposeall-in-one CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only