-
Plasmid#120045PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::PuroR-bGHpA
Plasmid#62440PurposeMXS_chaining vector with PGK::PuroR-bGHpADepositorInsertresistance cassette against Puromycin with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P17
Plasmid#120028PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-pheS_NOC
Plasmid#127031PurposeBasic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.DepositorInsertPart PheS_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationpheS A294G promiscuous mutationPromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet P2A Rac1-wt
Plasmid#125135PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.DepositorInsertmNeon/mScarletP2A-Rac1
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z06
Plasmid#120049PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::tTA2-bGHpA
Plasmid#62447PurposeMXS_chaining vector with CMV::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_MCS1
Plasmid#62395PurposeMXS_chaining vector with MCS1DepositorInsertEcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASP-HilA empty vector
Plasmid#153333PurposeTriggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressedDepositorTypeEmpty backboneUseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TGmiR_Luc-A
Plasmid#25765PurposeEntry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.DepositorInsertLuciferase miR-shRNA
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts[mCherry]-1A(lacZ)
Plasmid#126980PurposeLevel 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P32
Plasmid#120043PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHJ3: LexA-VHH-Control
Plasmid#108241PurposeLexA DNA binding domain fusing with anti-RNase A VHH antibodyDepositorInsertLexA DNA binding domain fusing with anti-RNase A VHH antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
IO201: pMVP (R4-R3) mCherry
Plasmid#121732PurposepMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg83-PDGFR_EF1a_iRFP670
Plasmid#186311PurposePlasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg83-PDGFR
iRFP670
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_P9-PDGFR_EF1a_mPlum
Plasmid#186307PurposePlasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.DepositorInsertsP9-PDGFR
mPlum
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP10-PDGFR_EF1a_mOrange
Plasmid#186308PurposePlasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line constructionDepositorInsertsAP10-PDGFR
mOrange
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only