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Plasmid#221473PurposeGateway cloning of NKX2-1 V205F DNA binding mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationV205FPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1
Plasmid#221472PurposeGateway cloning of NKX2-1 short isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Long
Plasmid#221483PurposeNKX2-1 long isoform lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1419
Plasmid#219778PurposepDEF-TurboID-GFPmut2, negative control for cytosolic expressionDepositorInsertTurboID/GFPmut2
UsePlasmodium, rodent malaria parasitesTagsExpressionMutationPromoterPbEF1alpha promoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD248
Plasmid#219498PurposeTemperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)DepositorInsertEco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 125-175
Plasmid#99668PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (125-175)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA(75-190)
Plasmid#99669PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTA(75-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (1-100)
Plasmid#99660PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-100)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (50-150)
Plasmid#99661PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (50-150)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 75-190
Plasmid#99663PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (75-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 75-175
Plasmid#99666PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (75-175)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 Full (1-261)
Plasmid#99654PurposeExpresses dSp Cas9 fused to p65 activation domainDepositorArticleInsertdCas9
UseTagsp65 activation domainExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (150-261)
Plasmid#99655PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (150-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (100-261)
Plasmid#99656PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (100-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (200-261)
Plasmid#99657PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (200-261)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (1-200)
Plasmid#99658PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-200)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 (1-150)
Plasmid#99659PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-150)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-P2A-3xFLAG-hEEA1 HDR template
Plasmid#214989PurposeEndogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.DepositorInsertEEA1 (EEA1 Human)
UseCRISPRTags3xFLAGExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SOD2(v1)
Plasmid#166214PurposeGateway entry vector for N. furzeri SOD2DepositorArticleInsertSOD2(v1)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only