-
Plasmid#90460PurposeRhoA mediated cell migrationDepositorInsertsUseTagsExpressionMammalianMutationR73Q and deletion a.a. (234-239)PromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pcDNA3.1-hRIPK1-Flag-K599R
Plasmid#112490PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationK599RPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-PGC-1alpha2
Plasmid#45502DepositorInsertPGC-1alpha Isoform2 (Ppargc1a Mouse)
UseTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4
Plasmid#112844Purposetransient/stable overexpression of the N-terminal and central domains of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationN + C1 domains of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP EMI1
Plasmid#158947PurposeExpress human EMI1 in mammalian cellsDepositorInsertEMI1 (FBXO5 Human)
UseTags2X FLAG 2X STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mouse cdk6 R31C
Plasmid#75171PurposeMammalian expression of mouse cdk6 with R31CDepositorInsertcyclin dependent kinase 6 (Cdk6 Mouse)
UseTagsExpressionMammalianMutationR31CPromoterCMVAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1hisA cdc25c C330S C377S
Plasmid#10965DepositorInsertcdc25c C330S C377S (CDC25C Human)
UseTags6xHisExpressionMammalianMutationC330S C377S. Cysteine to Serine substitution at …PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR17
Plasmid#128748PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-TTF-1-Q50K-Y54M
Plasmid#75264PurposeExpresses a double point mutant of human TTF-1DepositorInsertTTF-1 (NKX2-1 Human)
UseTagsnoneExpressionMammalianMutationQ50K and Y54M in the homeodomainPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse NCKX2-AS-long (pcDNA3.1+)
Plasmid#75199PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (Slc24a2 Mouse)
UseTagsExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE129 beta2AR Fz1-pcDNA3.1
Plasmid#16795DepositorUseXenopus expressionTagsExpressionMutationChimera of beta 2 adrenergic receptor (extracellu…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-K115R
Plasmid#112488PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationK115RPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human A3F-CTD
Plasmid#105052PurposeOverexpression of APOBEC3F C-terminal domain in mammalian cell linesDepositorInsertAPOBEC3F (A3F) C-terminal domain (APOBEC3F Human)
UseTagsHA tagExpressionMammalianMutationit contains only C terminal domain of A3FPromoterCMVAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-YFP
Plasmid#50483Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsYFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the YFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)
Plasmid#24653DepositorInsertSEK1 (MAP2K4 Human)
UseTagsHAExpressionMammalianMutationSer220Ala, Thr224LeuPromoterAvailable sinceJune 21, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
mOC-STAMP_pcDNA3.1 D/V5-His-TOPO
Plasmid#73448PurposeExpress mouse OC-STAMP in mammalian cellsDepositorInsertOcstamp (Ocstamp Mouse)
UseTagsV5-6xHisExpressionMammalianMutationN162DPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2
Plasmid#112842Purposetransient/stable overexpression of the central domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC1 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-BLMP-1-pcDNA3.1-
Plasmid#52514Purposeexpresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertblmp-1 (blmp-1 Nematode)
UseTags6xHisExpressionMammalianMutationN75S mutation compared GenBank reference sequence…PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only