Addgene

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMN-011

Plasmid

#195415

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF3 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-010

Plasmid

#195414

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF2

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF2 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-012

Plasmid

#195416

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF4

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF4 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-013

Plasmid

#195417

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-014

Plasmid

#195418

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF6

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF6 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-015

Plasmid

#195419

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF7

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF7 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-016

Plasmid

#195420

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF8

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF8 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-017

Plasmid

#195421

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF9

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF9 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-019

Plasmid

#195423

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF11

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF11 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV Sbf-Sal linker

Plasmid

#50434

Purpose

Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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InterTag-3xL

Plasmid

#186913

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)3-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
InterTag-2xL

Plasmid

#186912

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)2-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Gal4-RNF169UBD

Plasmid

#119013

Purpose

Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sg1-sg2

Plasmid

#192479

Purpose

All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Use

Tags

T2A mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-FUW-VdC9BV

Plasmid

#62195

Purpose

Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

Depositor

Kam Leong

Article

Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

Insert

VP64dCas9BFPVP64

Use

Lentiviral

Tags

Two VP64s tagged to dCas9 fused to BFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive) Cas9 (dCas9)

Promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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