-
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG244 iFAST
Plasmid#130809PurposeExpresses iFAST in mammalian cellsDepositorInsertiFAST
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TrxRFP2
Plasmid#172322Purposean improved fluorescent biosensor for thioredoxin redoxDepositorInsertTrxRFP2, an improved version from TrxRFP1
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
UseTagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME phluorin2-gpi
Plasmid#73793Purposeratiometric pHluorin2 with a gpi linkage sequence in a middle entry cloneDepositorInsertratiometric pHluorin2-gpi
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG247 His-iFAST
Plasmid#130808PurposeExpresses His-tagged iFAST in bacteriaDepositorInsertiFAST
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG328 td-iFAST
Plasmid#130811PurposeExpresses td-iFAST in mammalian cellsDepositorInserttd-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-TurboID
Plasmid#166570PurposeMultisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSSDepositorInsertTurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG330 td-FAST
Plasmid#130810PurposeExpresses td-FAST in mammalian cellsDepositorInserttd-FAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D
Plasmid#83131PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-GFP-C1 (w392-1)
Plasmid#17396PurposeGateway entry vector for N-term eGFP fusion, frame C1DepositorInsertGFP
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b
Plasmid#83129PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a
Plasmid#83166PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP11_pcDNA3.1(-)
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a G12D
Plasmid#83168PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBad-mKalama1
Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-mTagBFP2
Plasmid#96935PurposeRetroviral plasmid containing mTagBFP2 for labeling of cellsDepositorInsertMonomeric Tag Blue Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hs.NRAS G12V
Plasmid#83174PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutationDepositorInsertneuroblastoma RAS viral oncogene homolog (NRAS Human)
UseGateway entry cloneTagsExpressionMutationG12VPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only