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pcDNA3.1-N-Myc_DW164AA 1-205 USP7

Plasmid

#131254

Purpose

Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Expresses a mutant form of our pcDNA3.1-N-Myc_1-2…

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.mutant

Plasmid

#128746

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 point mutations disrupting the miR-7 site

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.miR16

Plasmid

#128747

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.miR17

Plasmid

#128748

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ArchT-BFP2-TSERex

Plasmid

#123312

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

ArchT-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y1175F-VEGFR2-mCherry

Plasmid

#139608

Purpose

Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Use

Tags

TEV-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Use

Tags

3xFLAG-TEV

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5H6-mPCSK3 D153A

Plasmid

#122681

Purpose

Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)

Use

Tags

Expression

Mammalian

Mutation

changed Aspartic Acid 153 to Alanine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD9-(TEV)-tTA

Plasmid

#162596

Purpose

human CD9-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD9 (CD9 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD63-(TEV)-tTA

Plasmid

#162597

Purpose

human CD63-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD63 (CD63 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-tTA

Plasmid

#162598

Purpose

human CD81-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614

Plasmid

#158074

Purpose

Expresses SARS-CoV-2 spike protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

D614G

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge