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Plasmid#37970DepositorInsertE2F3a (E2F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CAMK2D-T287A
Plasmid#221689PurposeMammalian expression of FLAG-tagged human CAMK2D (T287A)DepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationT287APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag S127A YAP
Plasmid#27370DepositorInsertYAP (YAP1 Human)
UseTagsflagExpressionMammalianMutationS127A ( major phosphorylation site in YAP2)PromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX
Plasmid#163572PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-NG-nCas9-UdgX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…PromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-ERK2-MEK1_fusion
Plasmid#39194DepositorUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV flag ERR alpha
Plasmid#10975DepositorInsertERR alpha (ESRRA Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD81-pHluorin
Plasmid#130903PurposeExpression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD81-pHluorin (CD81 aequorea victoria, Human)
UseTagspHluorinExpressionMammalianMutationpHluorin inserted between Leu-49 and Gly-50 in ex…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F4
Plasmid#37966DepositorInsertE2F4 (E2F4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
p212 pCMV-EGFP/RFP
Plasmid#8389DepositorInsertfloxed EGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F3b
Plasmid#37975DepositorInsertE2F3b (E2F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S94A
Plasmid#33102DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS94APromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-NGRN-Fc
Plasmid#115773PurposeExpression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertNGRN (NGRN Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHuji
Plasmid#130906PurposeExpression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHuji (CD9 Synthetic, Human)
UseTagspHujiExpressionMammalianMutationpHuji inserted between Asn50 and Asn51 in extrace…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCMV and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCMV4-3 HA/IkB-alpha(SS32,36AA)
Plasmid#24143DepositorInsertIkB alpha (NFKBIA Human)
UseTags3xHAExpressionMammalianMutationS32A S36APromoterAvailable sinceMarch 25, 2010AvailabilityAcademic Institutions and Nonprofits only