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Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceMay 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT3REVIN
Plasmid#111167PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralTagsDsRed and mVenusExpressionMutationWTPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VC155
Plasmid#194046PurposeTransient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VN154
Plasmid#194045PurposeTransient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-Sec22b
Plasmid#168029PurposeExpresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cellsDepositorInserteCFP-Sec22b(A131-H190)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-VAMP8
Plasmid#168028PurposeExpresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cellsDepositorInserteCFP-VAMP8(G7-R67)-mVenus
UseTagsHisx6 and mycExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK422
Plasmid#155373PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK423
Plasmid#155374PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO
Plasmid#68356Purpose-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK520
Plasmid#155386PurposePphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA1 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-BiFC
Plasmid#87856PurposepETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.DepositorInsertsEncodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus
UseTagsBiFC C-terminal fragmentExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO
Plasmid#68357PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.DepositorInserts5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
FlpO recombinase
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterU6 and synthetic Probasin ARRx2Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3a.H2B-K120C
Plasmid#224706PurposeExpresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamersDepositorInsertH2B-K120C (H2B Human)
UseTagsExpressionBacterialMutationK120CPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-858
Plasmid#218008PurposemVenus fluorescent proteinDepositorInsertmVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-854
Plasmid#218012PurposemVenus fluorescent proteinDepositorInsertmVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only