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Showing: 1441 - 1460 of 1656 results
  1. pJK503

    Plasmid
    #155380
    Purpose
    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    May 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pJK526

    Plasmid
    #155387
    Purpose
    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pGWT35S-AtOEP7(1-50aa)-VC155

    Plasmid
    #194046
    Purpose
    Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)
    Depositor
    Insert
    VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
    Use
    Tags
    AtOEP7 1–50aa
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pGWT35S-AtOEP7(1-50aa)-VN154

    Plasmid
    #194045
    Purpose
    Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)
    Depositor
    Insert
    VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
    Use
    Tags
    AtOEP7 1–50aa
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pJK422

    Plasmid
    #155373
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pJK423

    Plasmid
    #155374
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  11. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Use
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and synthetic Probasin ARRx2
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1460 of 1656 results