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RCAS-Neu HA SIINFEKL

Plasmid

#125832

Purpose

Retroviral expression of rat Neu cDNA

Depositor

Yi Li

Article

Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

Insert

Neu (Erbb2 Rat)

Use

Retroviral

Tags

2x HA and SIINFEKL

Expression

Mammalian

Mutation

Val to Glu point mutation of codon 664 and trunca…

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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XE120 CAMKII-K42M 1-271-CS2+

Plasmid

#16734

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-K42M 1-271 (Camk2a Rat)

Use

Xenopus expression

Tags

Expression

Mutation

C-terminal truncation of K42M kinase

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pBa.Halo-myosin Va tail 1006-1828

Plasmid

#162715

Purpose

Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Myosin Va (Myo5a Rat)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 1005 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPERIOR.puro-shREV3-4

Plasmid

#38029

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (REV3L Rat, human, Human, Mouse)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-rat ERK2-S151D

Plasmid

#40818

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

S151D

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD T325A T331A

Plasmid

#8973

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, T325A, T331A

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-parkin F463A

Plasmid

#45975

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F463A (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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mKO2-Lysosomes-20

Plasmid

#57880

Purpose

Localization: Lysosome Membrane, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Insert

LAMP1

Use

Tags

mKO2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pCRW0.8 p21 (rat)

Plasmid

#16477

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Use

Tags

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMT2 JNK3 p46

Plasmid

#12657

Depositor

John Kyriakis

Insert

JNK3 (Mapk10 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pBW105

Plasmid

#111137

Purpose

For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MLS-ratAANAT-3FLAG (Aanat Rat)

Use

Tags

3x Flag

Expression

Yeast

Mutation

Promoter

Gal1

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
FCIV1-GR S155A

Plasmid

#72702

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

S155A

Promoter

ubiquitin

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
Stx3-Dendra2

Plasmid

#82637

Purpose

Expresses fluorescent hybrid of syntaxin-3 for PALM imaging

Depositor

Janis Lochner

Insert

Syntaxin-3 (Stx3 Rat)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

HCMV

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only
mPlum-Cx43-7

Plasmid

#55961

Purpose

Localization: Gap Junctions, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

Cx43 (Gja1 Rat)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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p2HG/N795

Plasmid

#1284

Depositor

Susan Lindquist

Article

Picard et al Nature 1990 Nov 8;348(6297):166-8.

Insert

GR (Nr3c1 Rat)

Use

Tags

GPD promoter

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
FH95pUP95PDZGW (C6)

Plasmid

#74022

Purpose

lentiviral expression of Psd95 shRNA and Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

PDZ

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only