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Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA PSPC1 Y523F
Plasmid#124373PurposeExpresses PSPC1 Y523F in mammalian cellsDepositorInsertPSPC1 (PSPC1 Human)
UseTagsHA and HA tagExpressionMammalianMutationY523FPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2a
Plasmid#164963PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2a (kcnt2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-HqERV(del5'-LTR)
Plasmid#164089Purposetransfection of Hq-ERV without 5'-LTRDepositorInsertHq-ERV
UseEukaryotic expression vectorTagsExpressionMutationdeletion of 5'-LTRPromoternone (5'-LTR deleted)Available sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-HqERV(C1-20)
Plasmid#164090Purposetransfection of Hq-ERV with light-inducible promoterDepositorInsertHq-ERV
UseEukaryotic expression vectorTagsExpressionMutation5'-LTR replaced with light-regulatable C1-20…Promoterlight-activatable C1-20Available sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-Fah_S
Plasmid#163088Purposeexpressing mouse swingshift mutant FAH in bacterial cellsDepositorInsertFah_S
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_S
Plasmid#163086PurposeSwingshift mutant FAH in mammalian cellsDepositorInsertsFah_S
Fah_S
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-181-2xStrep
Plasmid#138444PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-181PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-191-2xStrep
Plasmid#138445PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-191PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only