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Showing: 1421 - 1440 of 15679 results

  1. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid
    #135260
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135260-plasmid-map-sequence-id-311150

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  2. ELL1 CtoA

    Plasmid
    #127279
    Purpose
    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
    Promoter
    EF1a
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127279-plasmid-map-sequence-id-249989

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  4. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid
    #73706
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    448-AAAA-451 and D143N
    Promoter
    CMV/tet
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  8. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207357-plasmid-map-sequence-id-408418

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  11. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171801-plasmid-map-sequence-id-337972

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  14. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid
    #171813
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171813-plasmid-map-sequence-id-338044

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  15. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  16. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

    Plasmid
    #171811
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-mgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171811-plasmid-map-sequence-id-337983

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  17. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid
    #171810
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171810-plasmid-map-sequence-id-337982

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  18. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid
    #171804
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-Ova-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171804-plasmid-map-sequence-id-337975

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  19. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid
    #171802
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-mgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171802-plasmid-map-sequence-id-337973

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Showing: 1421 - 1440 of 15679 results