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px330 p300 gRNA

Plasmid

#165591

Purpose

Insertion of p300 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Smarca2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pX335 HTT sgRNA-a

Plasmid

#87201

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001149414)

Plasmid

#75502

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BTK gRNA (BRDN0001149473)

Plasmid

#77074

Purpose

3rd generation lentiviral gRNA plasmid targeting human BTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BTK (BTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PLK1 G5.1 gRNA

Plasmid

#90834

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G5.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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PIP5K1C gRNA (BRDN0001145164)

Plasmid

#77667

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1C (PIP5K1C Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PI4K2A gRNA (BRDN0001147595)

Plasmid

#76822

Purpose

3rd generation lentiviral gRNA plasmid targeting human PI4K2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PI4K2A (PI4K2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pU6 SgRNA GAPDH

Plasmid

#162736

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

Spacer sequence for GAPDH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001148075)

Plasmid

#76891

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR - EGFP sgRNA 6

Plasmid

#51765

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 6

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB9337 (pgRNA_IV-1_NatMX)

Plasmid

#161590

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PDPK1 gRNA (BRDN0001146405)

Plasmid

#77453

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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CD2 gRNA (BRDN0001148522)

Plasmid

#75828

Purpose

3rd generation lentiviral gRNA plasmid targeting human CD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CD2 (CD2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CHUK gRNA (BRDN0001149372)

Plasmid

#77033

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CHUK gRNA (BRDN0001149269)

Plasmid

#77034

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHUK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHUK (CHUK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pX335 HTT sgRNA-b

Plasmid

#87200

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GSK3B gRNA (BRDN0001145264)

Plasmid

#77281

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR gRNA (BRDN0001149231)

Plasmid

#75690

Purpose

3rd generation lentiviral gRNA plasmid targeting human EGFR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge