-
Plasmid#212926PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD100
Plasmid#212924PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk3a
Plasmid#208985PurposeRiboprobe generationDepositorInsertkcnk3a (kcnk3a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk5a
Plasmid#208988PurposeRiboprobe generationDepositorInsertkcnk5a (kcnk5a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk6
Plasmid#208990PurposeRiboprobe generationDepositorInsertkcnk6 (kcnk6 Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk9
Plasmid#208992PurposeRiboprobe generationDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseCloningTagsExpressionMutationWithout STOP codonPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk13a
Plasmid#208996PurposeRiboprobe generationDepositorInsertkcnk13a (kcnk13a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IRdist_scram
Plasmid#162319PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationThe distal Upstream Motif site is scrambledPromoterpBADAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-N
Plasmid#210270PurposeN-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus R200W
Plasmid#209179PurposeMammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (R200W) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationNKX6-2 (R200W)PromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only