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pAAV-U6-gRNA-CBh-mCherry

Plasmid

#91947

Purpose

Expression of empty gRNA cassette and mCherry

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3

Plasmid

#136959

Purpose

Reporter vector encoding firefly luciferase and TurboRFP fluorescent protein

Depositor

Jakob Reiser

Article

Gills et al Oncotarget. 2018 Aug 24;9(66):32718-3272

Insert

Firefly luciferase and TurboRFP

Use

Lentiviral

Tags

V5 tag

Expression

Mutation

Promoter

CMV

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-All-in-one-hCascade

Plasmid

#134919

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lifeact-7

Plasmid

#54602

Purpose

Localization: Actin, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Type

Empty backbone

Use

Tags

Lifeact and mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2280

Plasmid

#111901

Purpose

ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple345 (NEFL MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA5

Plasmid

#26681

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 5 (KPNA5 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus C1

Plasmid

#27794

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus C1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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