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pGL3-noSV40-humanEC1.25_S3

Plasmid

#173957

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 3 (S3) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del8

Plasmid

#173980

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #8

Use

Luciferase

Tags

Expression

Mutation

Tiled deletion #8

Promoter

SV40 promoter

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(89-151)

Plasmid

#122299

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1 to 88

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(2-88)

Plasmid

#122298

Purpose

expresses a part of RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (partial) (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 89 to 151

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-RSLID1(WT)

Plasmid

#122296

Purpose

expresses RSL1D1 protein in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

vector's LTR

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP

Plasmid

#108394

Purpose

Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-3HA

Plasmid

#108398

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP

Plasmid

#108397

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP-3HA

Plasmid

#108396

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

Plasmid

#105681

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]

Plasmid

#153536

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

Syn

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2]

Plasmid

#195488

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-AURKB-PGK-mCherry

Plasmid

#153316

Purpose

Expresses AURKB and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

AURKB (AURKB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Myr-Flag-AKT1

Plasmid

#15294

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S218D S222D

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

Plasmid

#84446

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV1, AAV5, and AAV8

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-CyclinD1(WT)

Plasmid

#122300

Purpose

expresses Cyclin D1 protein with a Myc tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

CCND1 (CCND1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Jaws-KGC-GFP-ER2

Plasmid

#99233

Purpose

AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter. Using bGH pA signal.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

Plasmid

#84445

Purpose

AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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