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Plasmid#202237PurposeLevel 2 for strain-specific barcode DNA tag with bc100 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent markerDepositorInsertbc100
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC079
Plasmid#202238PurposeLevel 2 for strain-specific barcode DNA tag with bc106 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent markerDepositorInsertbc106
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC080
Plasmid#202239PurposeLevel 2 for strain-specific barcode DNA tag with bc107 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent markerDepositorInsertbc107
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC081
Plasmid#202240PurposeLevel 2 for strain-specific barcode DNA tag with bc108 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent markerDepositorInsertbc108
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC082
Plasmid#202241PurposeLevel 2 for strain-specific barcode DNA tag with bc109 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent markerDepositorInsertbc109
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC083
Plasmid#202242PurposeLevel 2 for strain-specific barcode DNA tag with bc110 through Tn7 bacterial insertion, Gentamicin selectable marker and GFP fluorescent markerDepositorInsertbc110
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC104
Plasmid#202243PurposeLevel 2 for strain-specific barcode DNA tag with bc131 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc131
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC105
Plasmid#202244PurposeLevel 2 for strain-specific barcode DNA tag with bc132 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc132
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC106
Plasmid#202245PurposeLevel 2 for strain-specific barcode DNA tag with bc133 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc133
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC107
Plasmid#202246PurposeLevel 2 for strain-specific barcode DNA tag with bc134 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc134
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-NlaCas7-p65
Plasmid#207394PurposeExpresses NlaCas7-p65 activator fusion in mammalian cellsDepositorInsertEF1a-NlaCas7-p65-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23109Available sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23117Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA009
Plasmid#216037PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorInsertNpNLS_v1.1; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA295
Plasmid#215986PurposeFragmid fragment: (N' terminus) SV40 Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; SV40NLS_v1.19
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA014
Plasmid#216057PurposeFragmid fragment: (2A Selection) Puromycin resistanceDepositorInsertP2A_v1.1; PuroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA163
Plasmid#216061PurposeFragmid fragment: (2A Selection) Stop onlyDepositorInsertStop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA082
Plasmid#215997PurposeFragmid fragment: (Selection) fluorescent reporterDepositorInsertStart; mTagBFP2_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only