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pGS-CD64-2

Plasmid

#109191

Purpose

Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Full-length engineered CD64, derived from human CD64

Use

Tags

Twin-Strep

Expression

Insect

Mutation

engineered for efficient expression, see publicat…

Promoter

Polyhedrin

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDule- Mj Acd A9

Plasmid

#197651

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Use

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep41

Plasmid

#136794

Purpose

Mammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep41 (CEP41 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZDeltaAL

Plasmid

#99551

Purpose

Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

replaced 1557-1618 with SGGSG

Promoter

T7

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3A

Plasmid

#89290

Purpose

For expression of the mammalian Atg8 protein LC3A without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3A (MAP1LC3A Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTRm1&2

Plasmid

#20880

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA p21 K161R, K163R

Plasmid

#78789

Purpose

To overexpress p21 K161R, K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K161R, K163R_muation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSK+ chickBMPR-1B CA

Plasmid

#49528

Purpose

cloning vector for constitutively active chick BMP receptor 1B

Depositor

Peter ten Dijke, Lee Niswander

Article

Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Insert

BMPR-1B CA (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

constitutively active mutation (Q203D); also P2A,…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pBabe 3xFLAG human Bmf

Plasmid

#17242

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
HOXB3 (human) FLAG pSP65

Plasmid

#8523

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX-6MYC p21

Plasmid

#78780

Purpose

To overexpress p21 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBabe 3xFLAG mouse Bmf

Plasmid

#17243

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
deltaVP1 + VP2C-GFP

Plasmid

#166676

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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NTRK1 gRNA (BRDN0001147322)

Plasmid

#75963

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK1 (NTRK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA_CDKN1A

Plasmid

#136523

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CDKN1A (CDKN1A Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K161Q, K163Q

Plasmid

#78790

Purpose

To overexpress p21 K161Q, K163Q in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K161Q, K163Q_muation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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His-GINA3

Plasmid

#74980

Purpose

Expresses His tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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NTRK1 gRNA (BRDN0001145717)

Plasmid

#75964

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK1 (NTRK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep41

Plasmid

#136843

Purpose

Mammalian expression of the centrosomal protein Cep41 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep41 (CEP41 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge