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Plasmid#105357PurposeSynthetic biologyDepositorInsertpromotor RPS5a (At3g11940; 1700bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG299
Plasmid#105360PurposeSynthetic biologyDepositorInsertpromotor AP1 (At1g69120;1400bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG295
Plasmid#105362PurposeSynthetic biologyDepositorInsertpromotor GILT (At4g12960;2100bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG300
Plasmid#105363PurposeSynthetic biologyDepositorInsertpromotor (At1g66850;350bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG025
Plasmid#105364PurposeSynthetic biologyDepositorInsertpromotor EDS1 (At3g48090)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-MCS-Avi
Plasmid#110205PurposeRecipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choiceDepositorInsertENH_BsmB1-MCS-Avi
UseTagsAvi_tagExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω1
Plasmid#101706PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α2
Plasmid#101707PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α1
Plasmid#101708PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω2
Plasmid#101709PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α2
Plasmid#101711PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MAM33MTS (12)
Plasmid#101693PurposeMAM33MTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG824
Plasmid#105401PurposeSynthetic biologyDepositorInsertSV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceMarch 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLhE_AW-pHG165 H118R
Plasmid#90063PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW/H118R
UseTagsExpressionBacterialMutationI48V/Q55A/Q60R/H118RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*-(ΔLF)
Plasmid#80045PurposeExpression of Vpr binding mutant EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationVpr binding site LF45 in p6 mutatedPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1+L2)
Plasmid#80051PurposeExpression of EPN-01 mutated in both L domainsDepositorInsertEPN-01(deltaPTAP/deltaYP)
UseTagsMycExpressionMammalianMutationPTAP10AAAA, YP37AAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔI)
Plasmid#80041PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationchanged Leu33 in I3-01 to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335_HR_Prnp_3
Plasmid#78622PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequenceDepositorInsertHR_Prnp_3 (Prnp Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM2
Plasmid#78625PurposeEncodes sgRNA2.0 targeting 138nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM1
Plasmid#78624PurposeEncodes sgRNA2.0 targeting 158nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB134
Plasmid#68588PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB434
Plasmid#68699PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB433
Plasmid#68698PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB334
Plasmid#68662PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29G37-end/pCMV-14-FLAG
Plasmid#73708PurposeExpress human TRAFD1 (FLN29) from G37 to end in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationdeletion of first 36 aa from full lenght TRAFD1PromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29 C60-end/pCMV-14-FLAG
Plasmid#73709PurposeExpress human TRAFD1 (FLN29) fromC60 to end in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationdeletion of first 59 aa from full lenght TRAFD1PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-C/pCMV-14-FLAG (Q391-END)
Plasmid#73590PurposeExpress C-terminal part (Q391- end) of human TRAFD1(FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-Mid/pCMV-14-FLAG (L175-D390)
Plasmid#73589PurposeExpress middle part (L175-D390) of human TRAFD1 in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLN29-N/pCMV-14-FLAG (1-I210)
Plasmid#73588PurposeExpress N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian cellsDepositorInsertTRAFD1 (TRAFD1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP32
Plasmid#71730Purposeinducible expression of TRP32 in E.coliDepositorInsertTRP32
UseTags6xHIS and ThioredoxinExpressionBacterialMutationPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATN (pKD-G12)
Plasmid#62691PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123/tdTomato
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
jam1a-pCRII
Plasmid#68803PurposeFor in situ antisense probe, NotI w/ Sp6DepositorInsertjam1a (f11r.1 Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pATL (pKD-G10)
Plasmid#62689PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only