pCMV-EPN-01*(ΔI)
(Plasmid
#80041)
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PurposeExpression of EPN-01 assembly mutant L33R
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV(WT)
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Backbone manufacturer...
- Backbone size w/o insert (bp) 5478
- Total vector size (bp) 6305
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPN-01*(L33R)
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SpeciesSynthetic
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Insert Size (bp)849
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Mutationchanged Leu33 in I3-01 to Ala
- Promoter CMV
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Tag
/ Fusion Protein
- Myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the L33R mutation is numbered according to the original I3-01 protein and is L50R when numbered according to EPN-01.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-EPN-01*(ΔI) was a gift from Wesley Sundquist (Addgene plasmid # 80041 ; http://n2t.net/addgene:80041 ; RRID:Addgene_80041) -
For your References section:
Designed proteins induce the formation of nanocage-containing extracellular vesicles. Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30. 10.1038/nature20607 PubMed 27919066