Addgene

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Use

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_026

Plasmid

#123741

Purpose

Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Flamindo2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1899)

Plasmid

#170143

Purpose

Expresses residues 1-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1900-2997

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-1899)

Plasmid

#170145

Purpose

Expresses residues 787-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-2138)

Plasmid

#170146

Purpose

Expresses residues 787-2138 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1547)

Plasmid

#170144

Purpose

Expresses residues 1-1547 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1548-2997

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (936-2997)

Plasmid

#170147

Purpose

Expresses residues 936-2997 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-935

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MTK0_030

Plasmid

#123969

Purpose

Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phiC31-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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