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Plasmid#222457PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-miniDFRAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00015
Plasmid#222446PurposeLevel 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.DepositorInsertVP64-NLS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91013
Plasmid#222450PurposeLevel 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.DepositorInsertCBS4-miniDFR::RUBY::OCS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91069
Plasmid#222462PurposeLevel 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-miniDFR (Solanum lycopersicum)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW101008
Plasmid#222453PurposeLevel 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.DepositorInsertCBS4-miniDFR::OsL5::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111101
Plasmid#222452PurposeLevel 1, Position 7 containing CBS4-miniDFR::Cre::NOS.DepositorInsertCBS4-miniDFR::Cre::NOS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91003
Plasmid#222449PurposeLevel 1, Position 3 containing 35S::RUBY::OCS.DepositorInsert35S::RUBY::OCS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91070
Plasmid#222463PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber Mosaic Virus)Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2696-CMV-Nme2SmuCas9-D56R
Plasmid#228717PurposeExpresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cellsDepositorInsertNme2SmuCas9-D56R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2697-CMV-Nme2SmuCas9-E932R
Plasmid#228718PurposeExpresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2SmuCas9-E932R nuclease
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00014
Plasmid#222445PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.DepositorInsertVP16-NLS
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag∆MA
Plasmid#212654PurposepiggyBac vector to express MLV Gag with truncated MA domainDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsExpressionMammalianMutationDeletion of MA domain (amino acids 2-215)PromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_CAG_Gag-MCP
Plasmid#212637PurposeLentivirus vector to express an MLV Gag-MCP fusion proteinDepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UseLentiviralTagsMS2 Capsid Protein (N55K)ExpressionMammalianMutationPromoterCAGAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110034
Plasmid#222443PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.DepositorInsertLoxP-mCherry
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Puro
Plasmid#227269PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only