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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE3G-c-fos-iRFP-B3-repRNA-A

Plasmid

#159926

Purpose

Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-358-iRFP670

Plasmid

#110694

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with iRFP670

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mutation

Truncation of CPSF6 isoform at amino acid 358

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-EGFP/RFP-int

Plasmid

#19818

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Type

Empty backbone

Use

Cre/Lox and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pLEX303 TagRFP-T LGALS3 R186S

Plasmid

#202428

Purpose

Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

TagRFP

Expression

Mutation

R186S

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-TAF15N-miRFP670-Cry2WT

Plasmid

#122440

Purpose

Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Use

Tags

IRFP and LEXY

Expression

Insect

Mutation

Promoter

maternal tubulin

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Use

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-RFP

Plasmid

#166843

Purpose

Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Use

Tags

RFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-CaURA3

Plasmid

#44882

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-mRFP

Plasmid

#117775

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe RFP-DN-FOXO1-HA neo

Plasmid

#45813

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA and RFP

Expression

Mammalian

Mutation

aa1-255

Promoter

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190076

Purpose

express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-cmlc2-eGFP

Plasmid

#117770

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP-miR-Scrint

Plasmid

#19825

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

Scrambled miRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pPRISM-Cre-gcry-mRFP

Plasmid

#117790

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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iRFP713-FRB-Mss4-Kina

Plasmid

#202744

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRTS-GBP-dCas9-mRFP

Plasmid

#102934

Purpose

Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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