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Showing: 3451 - 3500 of 30014 results

  9. AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

    Plasmid
    #190079
    Purpose
    express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
    Depositor
    Kenneth Matreyek
    Article
    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d
    Insert
    ACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190079-plasmid-map-sequence-id-371273

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  11. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166134
    Purpose
    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166134-plasmid-map-sequence-id-333283

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  27. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

    Plasmid
    #123324
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1
    Insert
    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123324-plasmid-map-sequence-id-239634

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  28. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

    Plasmid
    #123326
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1
    Insert
    shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123326-plasmid-map-sequence-id-239596

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  29. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

    Plasmid
    #123325
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1
    Insert
    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123325-plasmid-map-sequence-id-239636

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  30. pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

    Plasmid
    #123327
    Purpose
    Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1
    Insert
    shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123327-plasmid-map-sequence-id-239631

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  33. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  34. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138150-plasmid-map-sequence-id-350242

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  36. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  37. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  49. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-speā€¦
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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Showing: 3451 - 3500 of 30014 results