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Showing: 3451 - 3500 of 3976 results

  6. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  13. pDG03139

    Plasmid
    #195990
    Purpose
    Nanobody Sls-Nano2 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- SlsNano2-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195990-plasmid-map-sequence-id-385908

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  14. pDG03248

    Plasmid
    #195991
    Purpose
    Nanobody Sls-Nano2 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- SlsNano2-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195991-plasmid-map-sequence-id-385897

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  15. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  16. pHairyRed (double 35s removed)

    Plasmid
    #69710
    Purpose
    Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modified
    Depositor
    Peter Gresshoff, Brett Ferguson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    69710-plasmid-map-sequence-id-260035

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  17. QPRTA-c002

    Plasmid
    #205115
    Purpose
    QPRT protein expression
    Depositor
    Paul Brennan
    Insert
    QPRT (QPRT Human)
    Use
    Tags
    TEV-6His
    Expression
    Insect
    Mutation
    WT
    Promoter
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205115-plasmid-map-sequence-id-410409

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  18. SDC4A-c001

    Plasmid
    #205119
    Purpose
    SDC4 protein expression
    Depositor
    Paul Brennan
    Insert
    SDC4 (SDC4 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205119-plasmid-map-sequence-id-411015

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  19. pOpen-HSlig4

    Plasmid
    #165503
    Purpose
    Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DNA Ligase IV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165503-plasmid-map-sequence-id-338457

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  20. SLIT2A-c003

    Plasmid
    #205125
    Purpose
    SLIT2 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT2 (SLIT2 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205125-plasmid-map-sequence-id-412271

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  21. SLIT1A-c003

    Plasmid
    #205124
    Purpose
    SLIT1 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT1 (SLIT1 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205124-plasmid-map-sequence-id-412475

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  22. SDCBPA-c023

    Plasmid
    #205121
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205121-plasmid-map-sequence-id-411056

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  23. pLenti6.3_HES4

    Plasmid
    #124072
    Purpose
    HES4 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES4 (HES4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124072-plasmid-map-sequence-id-241438

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  24. SFRP1A-c500

    Plasmid
    #205122
    Purpose
    SFRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    SFRP1 (SFRP1 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205122-plasmid-map-sequence-id-412368

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  25. SFRP1A-c501

    Plasmid
    #205123
    Purpose
    SFRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    SFRP1 (SFRP1 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205123-plasmid-map-sequence-id-412370

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  26. TMEFF2A-c005

    Plasmid
    #205127
    Purpose
    TMEFF2 protein expression
    Depositor
    Paul Brennan
    Insert
    TMEFF2 (TMEFF2 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205127-plasmid-map-sequence-id-412277

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  27. TMEFF2A-c004

    Plasmid
    #205126
    Purpose
    TMEFF2 protein expression
    Depositor
    Paul Brennan
    Insert
    TMEFF2 (TMEFF2 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205126-plasmid-map-sequence-id-412272

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  28. SDCBPA-c012

    Plasmid
    #205120
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205120-plasmid-map-sequence-id-411022

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  33. PTNA-c200

    Plasmid
    #205114
    Purpose
    PTN protein expression
    Depositor
    Paul Brennan
    Insert
    PTN (PTN Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205114-plasmid-map-sequence-id-411200

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  35. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

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  36. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  39. pLenti6.3_HES2

    Plasmid
    #124070
    Purpose
    HES2 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES2 (HES2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    124070-plasmid-map-sequence-id-244596

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  40. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  41. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  42. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  43. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  44. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

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  45. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

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  46. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166089-plasmid-map-sequence-id-330353

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  47. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166086-plasmid-map-sequence-id-330352

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  48. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  49. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166090-plasmid-map-sequence-id-329612

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  50. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166092-plasmid-map-sequence-id-329611

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Showing: 3451 - 3500 of 3976 results