SDC4A-c001
(Plasmid
#205119)
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PurposeSDC4 protein expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 205119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHL-avitag3
- Backbone size w/o insert (bp) 4706
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSDC4
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SpeciesH. sapiens (human)
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MutationWT
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Entrez GeneSDC4 (a.k.a. SYND4)
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Tags
/ Fusion Proteins
- PTPRSsp (N terminal on insert)
- AviTag-6His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Encodes residues . Encodes ectodomain residues 19-145. Can be biotinylated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SDC4A-c001 was a gift from Paul Brennan (Addgene plasmid # 205119 ; http://n2t.net/addgene:205119 ; RRID:Addgene_205119)