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pMYH9-WT-V5

Plasmid

#183512

Purpose

Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMYH9-D1424H-FLAG

Plasmid

#183513

Purpose

Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 1424 to Histidine

Promoter

CMV

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only
pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KB

Plasmid

#32528

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KB (ITPKB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pGAPDH::H2B-mScarlet

Plasmid

#186764

Purpose

A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

H2B-mScarlet

Use

Overexpression

Tags

H2B

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IP3KA

Plasmid

#32529

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KA (ITPKA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Use

Tags

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-V5.hOr47a

Plasmid

#126474

Purpose

Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Insert

Or47a (Or47a Fly)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

codon optimization for H. sapiens

Promoter

CMV

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-V5.mER.hOr47a

Plasmid

#126473

Purpose

Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Insert

Or47a (Or47a Fly)

Use

Tags

H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

Expression

Mammalian

Mutation

codon optimization for H. sapiens

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNHT7::UMSBP::sNluc2_ORF1

Plasmid

#186765

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::ENO::sNluc2

Plasmid

#186770

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc0

Plasmid

#186771

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::hNluc

Plasmid

#186772

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::hNluc

Plasmid

#186774

Purpose

An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAPDH::sNluc1

Plasmid

#187221

Purpose

A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Overexpression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::YB1::sNluc1

Plasmid

#186754

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge