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Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET24a-His6-CUbo-MCS
Plasmid#212725PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-MCS
Plasmid#212724PurposeBacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-MCS-His6
Plasmid#212723PurposeBacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo-GFP
Plasmid#212720PurposeBacterial expression of His6-CUbo-GFPDepositorInsertGFP
UseTags6xHis-CUboExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His7-NUbo-GFP
Plasmid#212719PurposeBacterial expression of His7-NUbo-GFPDepositorInsertGFP
UseTags7xHis-NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-GFP-His6
Plasmid#212718PurposeBacterial expression of NUbo-GFP-His6DepositorInsertGFP
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-His6-TEV-Mms2
Plasmid#212716PurposeBacterial expression of His6-Mms2, His6-tag cleavable with TEV-proteaseDepositorInsertMms2
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)
Plasmid#99513PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationinternal inversion of nucleotides 265-312 of the …PromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-mCherry-FLAG
Plasmid#212798PurposeConstitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsmCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-FLAG
Plasmid#212797PurposeConstitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP-His6
Plasmid#212785PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-mCherry-VSV
Plasmid#212734PurposeConsitutive expression of CUbo-mCherry-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsmCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-VSV
Plasmid#212732PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-mCherry-VSV
Plasmid#212731PurposeConsitutive expression of NUbo-mCherry-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsmCherry-VSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-CUP1-NUbo-VSV
Plasmid#212730PurposeCopper-inducible expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterCUP1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-VSV
Plasmid#212729PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-VSV
Plasmid#212728PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastMutationPromoterADH1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-H2B-His8
Plasmid#212810PurposeConstitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cellsDepositorInsertH2B
UseTags8xHis and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only