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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_PCP-T5Exo

Plasmid

#214736

Purpose

encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

Depositor

Gil Westmeyer

Article

Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

Insert

CAG_PCP-T5Exo_bGH

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSB233

Plasmid

#164128

Purpose

PP7-CFP and PhlF-RFP expression plasmid

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

PP7-CFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AL12R

Plasmid

#161007

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Use

Tags

PP7 and H2B-mScarlet

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSB207

Plasmid

#164122

Purpose

J23101 driving PP7 repeats

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSB208

Plasmid

#164123

Purpose

Ptac driving PP7 repeats

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AL13Rb

Plasmid

#161006

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Use

Tags

PP7 and H2B-mScarlet

Expression

Plant

Mutation

Promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVW296

Plasmid

#139496

Purpose

Expression vector for the PP7-mCherry protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-mCherry tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW284

Plasmid

#139494

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pVW300

Plasmid

#139495

Purpose

Expression vector for the PP7-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pTEF PP7∆FG-GFPenvy tCYC1

Use

Tags

Expression

Yeast

Mutation

∆FG

Promoter

pTEF

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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AL13Rb-35S

Plasmid

#161008

Purpose

PP7-tagged reporter driven by the 35S promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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AL12R-HSP101

Plasmid

#161014

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AL12R-HSP70

Plasmid

#161010

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP70 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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AL13Rb-GAPC2

Plasmid

#161009

Purpose

PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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AL13Rb-HsfA2

Plasmid

#161012

Purpose

PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Use

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Mutation

Promoter

ponA

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP

Plasmid

#183934

Purpose

PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP

Use

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_INT

Plasmid

#68440

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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