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Showing: 121 - 140 of 461 results

  1. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  2. pAS-8 (seed1-8)

    Plasmid
    #40843
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–seed1-8
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pAS-7 (mm12-21)

    Plasmid
    #40842
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm12-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pAS-6 (mm17-21)

    Plasmid
    #40841
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm17-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pAS-5 (mm18-21)

    Plasmid
    #40840
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm18-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pAS-4 (mm19-21)

    Plasmid
    #40839
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm19-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pAS-3 (mm20-21)

    Plasmid
    #40838
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm20-21
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pAS-12 (mm1-8)

    Plasmid
    #40847
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Phillip Zamore
    Article
    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.
    Insert
    let-7–mm1-8
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. LeGO-iG2-wPRE-pA

    Plasmid
    #60489
    Purpose
    eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    IRES-eGFP; wPRE; BGH-pA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60489-plasmid-map-sequence-id-96860

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  13. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  14. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82703
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82703-plasmid-map-sequence-id-158053

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Showing: 121 - 140 of 461 results