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Plasmid#60242PurposeExpresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activitiesDepositorInsertNuclear Myosin 1 (Myo1c Mouse)
UseTagsEYFP and NLSExpressionMammalianMutationE407VPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AktAR2
Plasmid#184049PurposeNuclear-targeted ExRai-AktAR2.DepositorInsertNuc-ExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD434-SR mouse Alpha-synuclein
Plasmid#89073Purposebacterial expression of mouse Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
V5-BS2-SENP2 (Nuclear Pore)
Plasmid#220345PurposeMammalian expression vector BS2 in nuclear poreDepositorInsertBS2
UseTagsSENP2 (nuclear pore localized protein) and V5 tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
UseTagsNSP4 signal secretion peptide and mycExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-HA-myc-nuc (w557-1)
Plasmid#20025PurposeGateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)DepositorTypeEmpty backboneUseEntry vectorTagsHA, Myc, and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOTTC293 - pAAV EF1a V5-synuclein (WT)
Plasmid#60057PurposeAn AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.DepositorInsertalpha-synuclein (SNCA Human, human)
UseAAVTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-synORP1
Plasmid#191759PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-synORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-alpha-synuclein-BiFC
Plasmid#87855PurposepETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisationDepositorInsertsalpha-synuclein-VC155
alpha-synuclein-VN154
UseTagsC-terminal fragment of mVenus BiFC protein and N-…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-ORP1
Plasmid#191758PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-ORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nuc-mTurq2-Apollo-NADP+
Plasmid#193302PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the nucleusDepositorInsertG6PD (G6PD Human)
UseTagsSV40 NLS and mTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES EGFP
Plasmid#136618PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-F98-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
UseTagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease VIII E. coli
Plasmid#165561PurposeBifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.DepositorInsertEndonuclease VIII E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pD454-SR rat Alpha-synuclein
Plasmid#89074Purposebacterial expression of rat Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Rat)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L
Plasmid#52478PurposeExpresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-TDepositorInsertsEGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T
UseRabies virusTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A,H102A
Plasmid#159168PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A,H102A
UseTagsSV40 NLSExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only