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Showing: 301 - 350 of 7088 results

  1. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

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  2. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  6. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  7. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  8. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  9. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #42230
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42230-plasmid-map-sequence-id-419908

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  10. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  11. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  12. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  15. pCas9-EcRT-GAL-HIS3

    Plasmid
    #232093
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232093-plasmid-map-sequence-id-462313

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  16. pCas9-EcRT-GAL-Nat

    Plasmid
    #232090
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232090-plasmid-map-sequence-id-462306

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  17. pCas9-EcRT-GAL-Hyg

    Plasmid
    #232091
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232091-plasmid-map-sequence-id-462304

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  18. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  27. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

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  38. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  39. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122182-plasmid-map-sequence-id-237289

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  40. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid
    #122183
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    mNeonGreen
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122183-plasmid-map-sequence-id-241282

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  41. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid
    #122200
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9
    TagRFP-T
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122200-plasmid-map-sequence-id-236123

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  42. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  43. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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Showing: 301 - 350 of 7088 results