-
Plasmid#142226PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKB1 (NFKB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C423A-2xStrep
Plasmid#131111PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 423 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C399A-2xStrep
Plasmid#131112PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 399 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C393A-2xStrep
Plasmid#131113PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 393 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C355A-2xStrep
Plasmid#131114PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 355 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C344A-2xStrep
Plasmid#131115PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 344 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C341A-2xStrep
Plasmid#131116PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 341 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C301A-2xStrep
Plasmid#131117PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 301 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C298A-2xStrep
Plasmid#131118PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 298 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C295A-2xStrep
Plasmid#131119PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 295 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C289A-2xStrep
Plasmid#131120PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 289 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C244A-2xStrep
Plasmid#131121PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 244 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C427A-2xStrep
Plasmid#131109PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 427 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C424A-2xStrep
Plasmid#131110PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 424 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 200-429-2xStrep
Plasmid#130955PurposeExpresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 200-429PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha
Plasmid#15467DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha-KM
Plasmid#15468DepositorInsertIkB kinase alpha-KM (Chuk Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKbeta-KM
Plasmid#15466DepositorInsertIkB kinase beta-KM (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Myc-IKKalpha
Plasmid#19739DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKalphad17
Plasmid#19738DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationSplice variant lacking 17 amino acids at the C-te…PromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NFKB1_WT
Plasmid#82149PurposeGateway Donor vector containing NFKB1 , part of the Target Accelerator Plasmid Collection.DepositorInsertNFKB1 (NFKB1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK317
Plasmid#72472Purposepuc118 modified to contain ribosome binding siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterlacAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK635
Plasmid#72473PurposepET28a modified to contain TEV protease fusion tag instead of T7 tagDepositorTypeEmpty backboneUseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD619
Plasmid#66161Purposeheat-stable enterotoxin from parent strain Vibrio cholera NRT36SDepositorInsertnag-ST
UseCloning vectorTagsExpressionMutationPromoterUnknownAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV IkB alpha
Plasmid#12333DepositorInsertIkB alpha (Nfkbia Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
RCAS IkB alpha WT
Plasmid#12406DepositorInsertIkB alpha (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
FRP1864
Plasmid#159307PurposeYeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK515
Plasmid#72385PurposeProduces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)DepositorInsertphosphoribosylglycinamide formyltransferase 2
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK630
Plasmid#72389PurposeProduces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)DepositorInsertphosphoribosylpyrophosphate synthetase
UseTagsExpressionBacterialMutationinserted Val2PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK100
Plasmid#73264PurposeProduces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)DepositorInsertphosphoribosylformylglycinamidine synthase subunit PurS
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK683
Plasmid#72378PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic geneDepositorInsertphosphoribosyl pyrophosphate amidotransferase (PPAT Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK034
Plasmid#72383PurposeProduces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)DepositorInsertphosphoribosylglycinamide synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK649
Plasmid#72399PurposeProduces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)DepositorInsertamidophosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK210
Plasmid#73325PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioDDepositorInsertviolacein biosynthesis protein VioD
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK265
Plasmid#73598PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tagDepositorInsertviolacein biosynthesis protein VioC
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK261
Plasmid#73323PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tagDepositorInsertviolacein biosynthesis protein VioA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK678
Plasmid#72376PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic geneDepositorInsertphosphoribosyl pyrophosphate amidotransferase (PPAT Human)
UseGene synthesisTagsExpressionMutationPromotern/aAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK556
Plasmid#74277PurposeProduces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase
UseTagsHis6ExpressionBacterialMutationchanges alanine-1246 to serine, numerous silent m…PromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK196
Plasmid#73322PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tagDepositorInsertviolacein biosynthesis protein VioD
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK375
Plasmid#72410PurposeProduces Acetobacter aceti 1023 Est13DepositorInsertarylesterase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK379
Plasmid#72413PurposeProduces Acetobacter aceti 1023 Est14DepositorInsertarylesterase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK394
Plasmid#71703PurposeProduces Acetobacter aceti 1023 thioredoxin reductase 1 (AaTrxB1)DepositorInsertthioredoxin reductase 1
UseTagsExpressionMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK681
Plasmid#72271PurposeProduces Synechocystis PCC6803 orf sll1489 (synthetic gene)DepositorInserthypothetical protein SYNGTS_3005
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK588
Plasmid#72270PurposeSynthetic gene corresponding to Synechocystis PCC6803 orf sll1489DepositorInserthypothetical protein SYNGTS_3005
UseGene cloningTagsHis6 and TEV cleavage siteExpressionMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK709
Plasmid#72272PurposeExpression of Gluconacetobacter diazotrophicus succinyl-CoA:acetate CoA-transferase (synthetic gene)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK554
Plasmid#72453PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK694
Plasmid#72379PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic geneDepositorInsertphosphoribosyl pyrophosphate amidotransferase (PPAT Human)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK631
Plasmid#72390PurposeProduces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tagDepositorInsertphosphoribosylpyrophosphate synthetase
UseTagsHis6ExpressionBacterialMutationinserted Ala2 to facilitate cloningPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
UseTagsExpressionYeastMutationmissing residues 1-6PromoterGAL1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK373
Plasmid#72408PurposeProduces Acetobacter aceti 1023 Est03DepositorInsert4-hydroxybenzoyl-CoA thioesterase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only