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Showing: 301 - 350 of 2203 results
  1. TFORF0723

    Plasmid
    #142226
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    NFKB1 (NFKB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid
    #131111
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid
    #131112
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid
    #131113
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA4/TO-ORF66 C355A-2xStrep

    Plasmid
    #131114
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 355 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA4/TO-ORF66 C344A-2xStrep

    Plasmid
    #131115
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 344 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA4/TO-ORF66 C341A-2xStrep

    Plasmid
    #131116
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 341 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA4/TO-ORF66 C301A-2xStrep

    Plasmid
    #131117
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 301 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA4/TO-ORF66 C298A-2xStrep

    Plasmid
    #131118
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 298 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA4/TO-ORF66 C295A-2xStrep

    Plasmid
    #131119
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 295 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA4/TO-ORF66 C289A-2xStrep

    Plasmid
    #131120
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 289 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA4/TO-ORF66 C244A-2xStrep

    Plasmid
    #131121
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 244 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA4/TO-ORF66 C427A-2xStrep

    Plasmid
    #131109
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 427 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA4/TO-ORF66 C424A-2xStrep

    Plasmid
    #131110
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 424 muta…
    Promoter
    CMV
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pJK317

    Plasmid
    #72472
    Purpose
    puc118 modified to contain ribosome binding site
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  17. pCVD619

    Plasmid
    #66161
    Purpose
    heat-stable enterotoxin from parent strain Vibrio cholera NRT36S
    Depositor
    Insert
    nag-ST
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  18. CMV IkB alpha

    Plasmid
    #12333
    Depositor
    Insert
    IkB alpha (Nfkbia Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
  19. RCAS IkB alpha WT

    Plasmid
    #12406
    Depositor
    Insert
    IkB alpha (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
  20. FRP1864

    Plasmid
    #159307
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized tdKOkappa under KanMX selection
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  21. pJK515

    Plasmid
    #72385
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)
    Depositor
    Insert
    phosphoribosylglycinamide formyltransferase 2
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  22. pJK630

    Plasmid
    #72389
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)
    Depositor
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  23. pJK100

    Plasmid
    #73264
    Purpose
    Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)
    Depositor
    Insert
    phosphoribosylformylglycinamidine synthase subunit PurS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. pJK683

    Plasmid
    #72378
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  25. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Insert
    phosphoribosylglycinamide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  26. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  27. pJK210

    Plasmid
    #73325
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD
    Depositor
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  28. pJK265

    Plasmid
    #73598
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    Insert
    violacein biosynthesis protein VioC
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  29. pJK261

    Plasmid
    #73323
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tag
    Depositor
    Insert
    violacein biosynthesis protein VioA
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  30. pJK678

    Plasmid
    #72376
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  31. pJK556

    Plasmid
    #74277
    Purpose
    Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)
    Depositor
    Insert
    formylglycinamidine ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-1246 to serine, numerous silent m…
    Promoter
    T7
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  32. pJK196

    Plasmid
    #73322
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  33. pJK375

    Plasmid
    #72410
    Purpose
    Produces Acetobacter aceti 1023 Est13
    Depositor
    Insert
    arylesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  34. pJK379

    Plasmid
    #72413
    Purpose
    Produces Acetobacter aceti 1023 Est14
    Depositor
    Insert
    arylesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  35. pJK394

    Plasmid
    #71703
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 (AaTrxB1)
    Depositor
    Insert
    thioredoxin reductase 1
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  36. pJK681

    Plasmid
    #72271
    Purpose
    Produces Synechocystis PCC6803 orf sll1489 (synthetic gene)
    Depositor
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  37. pJK588

    Plasmid
    #72270
    Purpose
    Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489
    Depositor
    Insert
    hypothetical protein SYNGTS_3005
    Use
    Gene cloning
    Tags
    His6 and TEV cleavage site
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  38. pJK709

    Plasmid
    #72272
    Purpose
    Expression of Gluconacetobacter diazotrophicus succinyl-CoA:acetate CoA-transferase (synthetic gene)
    Depositor
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  39. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  40. pJK694

    Plasmid
    #72379
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  41. pJK631

    Plasmid
    #72390
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag
    Depositor
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    inserted Ala2 to facilitate cloning
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  42. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  43. pJK373

    Plasmid
    #72408
    Purpose
    Produces Acetobacter aceti 1023 Est03
    Depositor
    Insert
    4-hydroxybenzoyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 350 of 2203 results