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Showing: 241 - 280 of 352 results

  3. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid
    #182305
    Purpose
    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.
    Depositor
    Andrew York
    Article
    Seidel et al (2021), doi: 10.5281/zenodo.5810930
    Insert
    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    Use
    Tags
    A modified TorA periplasmic targeting sequence pl…
    Expression
    Bacterial
    Mutation
    Spontaneous mutation of calmodulin's penulti…
    Promoter
    t7
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    182305-plasmid-map-sequence-id-360536

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  33. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111026-plasmid-map-sequence-id-214044

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  37. pFLIP43

    Plasmid
    #65618
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65618-plasmid-map-sequence-id-111420

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  38. pFLIP38

    Plasmid
    #65616
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edCit-attR1 and attR2-edCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65616-plasmid-map-sequence-id-111417

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  39. pFLIP37

    Plasmid
    #65505
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-Cit-attR1 and attR2-Cer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65505-plasmid-map-sequence-id-111225

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  40. pFLIP30

    Plasmid
    #65499
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65499-plasmid-map-sequence-id-111219

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Showing: 241 - 280 of 352 results