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Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only
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pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MLS-HyPer7
Plasmid#136470PurposeMammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsTandem mitochondrial targeting signal of cytochro…ExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#201157PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a-VP64
UseCRISPR and LentiviralTagsExpressionMutationD908APromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE2max-∆RNaseH-dualU6
Plasmid#198735PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettesDepositorInsertNpuC-CtermPE2max∆RNaseH
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-PIP-FUCCI
Plasmid#138715PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRGFP
Plasmid#26475PurposeIn vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection markerDepositorInsertDRGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR' CMV GFP
Plasmid#14858DepositorInsertCMV-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
Plasmid#222993PurposeMammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-His
Plasmid#218190PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
UseTagsHis and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D1R
Plasmid#89160PurposeExpress vaccinia virus D1R in mammalian cellsDepositorInsertVaccinia virus D1R
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-RanQ69L
Plasmid#30309DepositorInsertRanQ69L (RAN N/A)
UseTagsFLAG-CherryExpressionMammalianMutationGlutamine 69 is changed to LeucinePromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D12L
Plasmid#89161PurposeExpress vaccinia virus D12L in mammalian cellsDepositorInsertVaccinia virus D12L
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1
Plasmid#121478Purposeexpression of human PD-L1 in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only