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Showing: 1361 - 1380 of 3155 results

  1. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

    Plasmid
    #224883
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP125-160 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP125-160
    Promoter
    CUP1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224883-plasmid-map-sequence-id-448180

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  2. pRS416 CUP1pro-M-PRR-Htt97QP4-YFP

    Plasmid
    #224885
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.
    Depositor
    Paul Herman
    Insert
    M-PRR-Htt97QP4 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    N-PRR
    Promoter
    CUP1
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    224885-plasmid-map-sequence-id-448159

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  3. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

    Plasmid
    #224880
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    Htt103QdeltaP141-170 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    deltaP141-170
    Promoter
    CUP1
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    224880-plasmid-map-sequence-id-448151

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  4. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

    Plasmid
    #224876
    Purpose
    Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.
    Depositor
    Paul Herman
    Insert
    HTT103QdeltaP161-170 (HTT Human)
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    delta161-170
    Promoter
    CUP1
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    224876-plasmid-map-sequence-id-448047

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  6. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  9. pLenti-hSyn1-ChR2(H134R)-EYFP

    Plasmid
    #197593
    Purpose
    Lentiviral transfer vector for the neuronal expression of ChR2(H134R)-EYFP (Channelrhodopsin for the inducing neuronal activity)
    Depositor
    Patrik Verstreken
    Insert
    ChR2(H134R)-EYFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197593-plasmid-map-sequence-id-389346

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  12. pEYFP-N1-HsSmg5-del810-846-siRNAres_I

    Plasmid
    #146539
    Purpose
    Mammalian Expression of HsSmg5-del810-846-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del810-846-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146539-plasmid-map-sequence-id-288985

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  13. pEYFP-N1-HsSmg5-del847-1017-siRNAres_I

    Plasmid
    #146540
    Purpose
    Mammalian Expression of HsSmg5-del847-1017-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del847-1017-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146540-plasmid-map-sequence-id-288986

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  14. pEYFP-N1-HsSmg5-del13-266-siRNAres_I

    Plasmid
    #146537
    Purpose
    Mammalian Expression of HsSmg5-del13-266-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del13-266-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    two non silent mutation, T980C (L327P), T1084C (C…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146537-plasmid-map-sequence-id-288983

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  18. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

    Plasmid
    #160583
    Purpose
    Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    YFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    160583-plasmid-map-sequence-id-338464

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Showing: 1361 - 1380 of 3155 results