-
Plasmid#204733PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR1_ccC
Plasmid#158115Purposedeletion hADAR1DepositorInsertAdar1 (ADAR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR1_ccB
Plasmid#158116Purposedeletion hADAR1DepositorInsertAdar1 (ADAR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro Ras V12
Plasmid#1768DepositorInsertras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FLAG-dTomato-centrin-1
Plasmid#73332PurposeLentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tagDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsFLAG and dTomatoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mu-FLT3Lv1-pTRIP
Plasmid#163519PurposeUsed to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cellsDepositorInsertmurine-FLT3L variant 1 (Flt3l Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-CTSS-puro
Plasmid#158469PurposeConstitutive lentiviral expression of human CTSS.DepositorInsertCTSS (CTSS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-4EBP1-T37A/T46A/Y54A/L59A
Plasmid#136421PurposeStable overexpression of elF4EBP1 T37A/T46A/Y54A/L59ADepositorInserteIF4EBP1 (EIF4EBP1 Human)
UseLentiviralTagsExpressionMammalianMutationT37A/T46A/Y54A/L59APromoterCMVAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5-TurboID_IDG-K
Plasmid#135241PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSicoR shluc
Plasmid#14782DepositorInsertLuciferase shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FLAG-mCherry-C1-centrin-1
Plasmid#73333PurposeLentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tagsDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 hPHGDH
Plasmid#154916PurposeDoxycyclin inducible expression of human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK93_Lifeact-mCherry
Plasmid#46357DepositorInsertLifeact
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpBABEAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAID5.1-N
Plasmid#145808PurposeAn all-in-one bicistronic plasmid for controlling protein degradation by AID technologyDepositorTypeEmpty backboneUseTagsmAIDExpressionMammalianMutationPromoterAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PtenC124S-Puro
Plasmid#135677PurposeConstitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationC124SPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215008PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-eGFP_IDG-K
Plasmid#135263PurposeGateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-eGFP
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only