-
Plasmid#226501PurposeSuperslow-decay iGluSnFR3 variant 1 for population imagingDepositorInsertiGluSnFR3 D25A
UseTagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationD25APromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-iGlu3Fast
Plasmid#226500PurposeUltrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate releaseDepositorInsertiGluSnFR3 S72T
UseTagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationS72TPromoterCMVAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-iGlu3slow2
Plasmid#226502PurposeSuperslow-decay iGluSnFR3 variant 2 for population imagingDepositorInsertiGluSnFR3 D25R
UseTagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationD25RPromoterCMVAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB401
Plasmid#209130PurposeMultipartite assembly of the TU for afpA expression under the regulation of afpA promoter and terminator with the pyr4 auxotrophy marker pyr4 from T. reseeiDepositorInsertPafpA:afpA:TafpA::Ppyr4:pyr4:Tpyr4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-rabbit-Caspase-4
Plasmid#183375PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb-mCherry
Plasmid#118753PurposeMultisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFFDepositorInsertccdb
UseTagsmCherryExpressionMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb
Plasmid#141067PurposeMultisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBODepositorInsertccdb
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb-EGFP
Plasmid#118752PurposeMultisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEXDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-mCherry-ccdb
Plasmid#118629PurposeMultisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZDepositorInsertccdb
UseTagsmCherryExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-ccdb
Plasmid#118628PurposeMultisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7gRNA-ccdb
Plasmid#140864PurposeBackbone for making sgRNAs.DepositorInsertccdb/CAM
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab37
Plasmid#140886PurposeExpression of zebrafish GFP-tagged Rab37 in mammalian cells.DepositorInsertRab37
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CaaX(tH) in pCSDEST2
Plasmid#140890PurposeExpression of GFP-CaaX (sequence from Hras) in mammalian cells.DepositorInsertCaaX membrane targeting motif (H-ras)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI743 dual-barcoded gRNA parent vector
Plasmid#164915PurposeCEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid librariesDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI733 pRPR1(TetO)-RPC31-sgRNA
Plasmid#164913PurposeFor yeast genomic integration of sgRNA against RPC31DepositorInsertpRPR1(TetO)-RPC31-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterRPR1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI732 pRPR1(TetO)-HTS1-sgRNA
Plasmid#164912PurposeFor yeast genomic integration of sgRNA against HTS1DepositorInsertpRPR1(TetO)-HTS1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterRPR1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
p3E-rab38a
Plasmid#109699PurposeMultisite gateway vector for 3' tagging with rab38aDepositorInsertrab38a (rab38a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab32b
Plasmid#109690PurposeMultisite gateway vector for 3' tagging with rab32bDepositorInsertrab32b (rab32b Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only