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Plasmid#40281DepositorInsert2C Promoter (2-cell stage promoter)
UseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-GFP11
Plasmid#78590PurposeExpression flag-GFP 11 in mammalian cellsDepositorInsertflag-GFP 11
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GCamp6-3NLS
Plasmid#165401PurposeExpresses a nuclear localized genetically encoded calcium reporter.DepositorInsertNuclear Localization Signal
UseAvian, xenopus, zebrafishTagsGCamp6ExpressionMammalianMutationPromoterSP6Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSCX
Plasmid#63918PurposeExpression of the sucrose utilization phenotypeDepositorInsertcscAKB
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-mLanYFP-polyA
Plasmid#174021PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmLanYFP
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMA2641
Plasmid#25435DepositorInsertreverse tetracycline-regulated transactivator advanced
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-tdTomato-polyA
Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-myc-T58A-S62D
Plasmid#138540PurposeFor making myc-T58A-S62D RNADepositorInsertMyelocytomatosis oncogene T58A S62D (Myc Mouse)
UseIn vitro transcriptionTagsExpressionMutationThreonine 58 changed to alanine and serine 62 cha…PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCM-HA-Gas1
Plasmid#38313DepositorInsertGas1 (GAS1 Budding Yeast)
UseTags2xHAExpressionMutationcontains promoter regionPromoterEndogenousAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pICXNHG3
Plasmid#49021PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterAOX1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pitx1
Plasmid#84319PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pitx1. Not for gene expression.DepositorInsertPitx1 (Pitx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-BarcodeV5
Plasmid#78629PurposeGESTALT V5 integrating barcode with Puro and EGFPDepositorInsertGESTALT lineage tracing target V5
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIRT5H158Y-pET15b
Plasmid#81032PurposeBacterial expression of Human catalytic mutant SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
UseTagsHisExpressionBacterialMutationH158Y catalytic mutantPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA
Plasmid#47069PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), AVI-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
RHPMP133 (mOrange2-beta Actin)
Plasmid#88837PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mOrange2DepositorInsertbeta-Actin (ACTB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBXCA3GH
Plasmid#47071PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, GFP…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ub[9]-mRFP[1]
Plasmid#20249DepositorInsertubiquitin C (UBC Human)
UseTagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycinePromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFT1.0
Plasmid#46138DepositorInsertmCherry
UseCre/Lox; Mutagenic gene trap for zebrafishTagsExpressionMutationPromoterAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
RHPMP132 (mEOS3.1-beta Actin)
Plasmid#88836PurposeLocalization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1DepositorInsertbeta-Actin (ACTB Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only