1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. mCherry-C1-TGAT

   Plasmid

   #84334

   Purpose

   Expresses TGAT tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   TGAT (TRIO Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPMVAugw

   Plasmid

   #98300

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TEFM1EfmvaE>TEBS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CAG-IntCCCre-T2A-CopGFP

   Plasmid

   #160501

   Purpose

   Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   IntCCCre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   CopGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. amilCP chromoprotein promoter-less

   Plasmid

   #118008

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PliaI

   Plasmid

   #55177

   Purpose

   Bacitracin-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   liaI promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   liaI

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

    Plasmid

    #97010

    Purpose

    Rosa26 targeting vector for FLP-responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB21_TaNAM-A1

    Plasmid

    #124572

    Purpose

    Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Tags

    10xMyc

    Expression

    Plant

    Mutation

    Promoter

    35s

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-GFP RanSen

    Plasmid

    #184705

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

    Plasmid

    #160507

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p35S::mCh-HA-P2A-lifeact-mCh

    Plasmid

    #135234

    Purpose

    constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    mCh-HA-P2A-lifeact-mCh
    

    Use

    Tags

    HA, lifeact, and p2a

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRW006-SM-destination-pro35S-Cas9

    Plasmid

    #104439

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PliaG

    Plasmid

    #55174

    Purpose

    Constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    LiaG promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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