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pFOSGE_COF4C06

Plasmid

#71047

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED19 (MED19 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
psiCheck2 3xUTR

Plasmid

#40762

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

TK/SV40

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
psicheck2 6xUTR

Plasmid

#40763

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

TK/SV40

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBXNAC3H

Plasmid

#47074

Purpose

FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJT87

Plasmid

#204489

Purpose

CAG promoted WBeta recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

WBeta recombinase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJT88

Plasmid

#204490

Purpose

CAG promoted phiC31 recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

phiC31 recombinase

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CAG

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ301

Plasmid

#205415

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Hs1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-gR082

Plasmid

#188551

Purpose

Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR082

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-gR085

Plasmid

#188552

Purpose

Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA* w ccdB

Plasmid

#192310

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-Linker

Plasmid

#192305

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBM10a

Plasmid

#179688

Purpose

Target plasmid for context profiling

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

ACA target

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL7006

Plasmid

#136945

Purpose

Malachite green aptamer, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Tags

Expression

Mutation

Promoter

J23119

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRC03

Plasmid

#114682

Purpose

DHFR-sfGFP.Strand11

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

DHFR-sfGFP.Strand11

Use

Tags

GST

Expression

Mutation

Promoter

T7 Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDA199

Plasmid

#74275

Purpose

pSTL1-dPSTRy(4,3)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRy(4,3)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3G02

Plasmid

#70955

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG16952 (CG16952 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1C08

Plasmid

#70827

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG12081 (CG12081 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only