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pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

Plasmid

#181850

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD51 (Mief1 Mouse)

Use

Tags

4xMyc and 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tau Authentic Alu 10-12V337M

Plasmid

#194169

Purpose

Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 V337M

Use

Tags

3X flag

Expression

Mammalian

Mutation

Changed Valine 337 to Methinonine

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-888 USP7

Plasmid

#131248

Purpose

Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_884-1102 USP7

Plasmid

#131249

Purpose

Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y1214F-VEGFR2-mCherry

Plasmid

#139609

Purpose

Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P-Kv

Plasmid

#179462

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/myc-his Ndel1

Plasmid

#12572

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Clover-Prx2-mRuby2

Plasmid

#117906

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (WT)

Plasmid

#73963

Purpose

STAG2 cDNA (CCDS43990) wild type

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

wild type

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CD9-(TEV)-tTA

Plasmid

#162596

Purpose

human CD9-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD9 (CD9 Human)

Use

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG

Plasmid

#35981

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Use

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 101 amino acids

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDO2-p2a-eGFP

Plasmid

#187027

Purpose

Expresses IDO2 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Article

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 2 (IDO2 Human)

Use

Tags

P2A-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ GEMIN6 myc HIS

Plasmid

#71659

Purpose

Mammalian expression of GEMIN6

Depositor

Gideon Dreyfuss

Insert

GEMIN6 (GEMIN6 Human)

Use

Tags

HIS and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-2xFLAG-2xSTREP-CCND1

Plasmid

#172648

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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